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Prasad, K. V. S. R. G.
- Protective Effect of Rutin against Cisplatin-induced Nephrotoxicity in Rats
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PDF Views:425
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Journal of Natural Remedies, Vol 10, No 2 (2010), Pagination: 144-151Abstract
Objective: To study the nephroprotector activity of the bioflavonoid, rutin (50 and 100 mg/kg, p.o.) on cisplatininduced nephrotoxicity (6mg/kg, i.p.) in albino rats. Materials and Methods: Nephroprotector activity of rutin was assessed by estimating the levels of blood urea nitrogen, serum creatinine, serum total proteins, urinary protein, creatinine clearance, urine to serum creatinine ratio and lipid peroxidation in kidney. Results: Cisplatin elevated the serum markers level, increased the protein excretion in urine, reduced the creatinine clearance, urine to serum creatinine ratio and renal malondialdehyde level was increased. Animals which received rutin, in curative and prophylactic groups significantly reversed the effects induced by cisplatin. Conclusion: From the results it is revealed that rutin effectively reduced the cisplatin-induced renal toxicity in albino rats.Keywords
Cisplatin, Nephrotoxicity, Rutin, Nephroprotector Activity- Hepatoprotective and Antioxidant Activity of Methanolic Extract of Actinodaphne madraspatana Against Carbon Tetrachloride Induced Hepatotoxicity
Abstract Views :359 |
PDF Views:2
Authors
Affiliations
1 Department of Biotechnology, Acharya Nagarjuna University, Guntur, IN
2 Department of Pharmaceutical Sciences, Sri Padmavathi Mahila Visvavidyalayam, Tirupathi, IN
3 Department of Pharmacology, Malla Reddy Institute of Pharmaceutical Sciences, Secundrabad, IN
1 Department of Biotechnology, Acharya Nagarjuna University, Guntur, IN
2 Department of Pharmaceutical Sciences, Sri Padmavathi Mahila Visvavidyalayam, Tirupathi, IN
3 Department of Pharmacology, Malla Reddy Institute of Pharmaceutical Sciences, Secundrabad, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 6, No 4 (2014), Pagination: 176-180Abstract
The present study deals with the investigation of the hepatoprotective and antioxidant effect of methanolic extract of Actinodaphne madraspatana. Biochemical parameters like Serum Glutamate Oxaloacetate transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT), Alkaline phosphatase (ALP), serum triglycerides and serum bilirubin were measured. The administration of single dose of 1.25 ml/kg of carbon tetrachloride cause an increase in the serum levels of estimated biochemical parameters whereas group of rats pretreated orally with silymarin (10 mg/kg) and methanolic extracts (200 and 400 mg/kg) of Actinodaphne madraspatana for five consecutive days showed a decrease in the serum levels of biochemical parameters. Histopathological assessment were also done. Histological analysis revealed that methanolic extract of Actinodaphne madraspatana (200 and 400 mg/kg) and silymarin reduced the incidence of liver lesions, central vein necrosis, apoptosis, hepatocellular vascuolization and fatty changes caused by CCl4. The extract significantly elevated the levels catalase, reduced glutathione and declined the levels of malondialdehyde in liver, as compared to those in CCl4 treated group and suggests that the hepatoprotective effect of Actinodaphne madraspatana is due to the antioxidant effect of the extract. Phytochemical analysis of methanolic extract of A. madraspatana revealed the presence of alkaloids, glycosides, tannins, flavonoids and carbohydrates.Keywords
Actinodaphne madarasapatana, Hepatoprotective, Silymarin, SGOT, SGPT, ALP, Serum Total Bilirubin, Serum Triglycerides.References
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- Biotransformation of Paracetamol by Cunninghamella echinulata
Abstract Views :164 |
PDF Views:72
Authors
Affiliations
1 Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam (Women's University), Tirupati - 517 502, Andhra Pradesh, IN
1 Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam (Women's University), Tirupati - 517 502, Andhra Pradesh, IN
Source
Journal of Pharmaceutical Research, Vol 8, No 1 (2009), Pagination: 1-5Abstract
The aim of the study was to develop a microbial model for synthesis of paracetamol metabolite for further pharmacological and toxicological studies. The metabolite of paracetamol in microbial cultures was identified, isolated and confirmed using fermentation techniques, Thin Layer Chromatography (TLC) and High Pressure Liquid Chromatography (HPLC) followed by liquid chromatography/mass spectrometry (LC/MS). Among different organisms screened, Cunninghamella echinulata showed an extra peak at 5.1 min in HPLC compared to its controls indicating formation of a metabolite. The metabolite was further characterized by mass spectrometry and was found to be N-acetyl-p-benzoquinoneimine (NAPQI) which is a toxic metabolite. Cunninghamella echinulata was able to metabolize paracetamol to its toxic metabolite by N-hydroxylation and rearrangement similar to human beings. This study has developed a model to produce toxic metabolites of other similar drugs easily for further toxicological and pharmacological studies.Keywords
Microbial Model, Metabolism, Paracetamol, N-Hydroxylation, NAPQI.- Evaluation of Ruellia tuberosa L. for Antiurolithiatic and Antioxidant Activities in Rats
Abstract Views :160 |
PDF Views:78
Authors
Affiliations
1 Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam (Women’s University), TIRUPATI-517 502, IN
1 Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam (Women’s University), TIRUPATI-517 502, IN
Source
Journal of Pharmaceutical Research, Vol 9, No 2 (2010), Pagination: 70-75Abstract
The ischolar_mains of Ruellia tuberosa L. are recommended for kidney stone disorders in the Indian traditional system of medicine. Ethanolic extract of R. tuberosa ischolar_mains was evaluated for antiurolithiatic activity against 0.75% v/v ethylene glycol and 2% w/v ammonium chloride induced calcium oxalate urolithiasis and for antioxidant activity against hyperoxaluria induced oxidative stress in male albino rats. Ethylene glycol and ammonium chloride administration increased the deposition of calcium and oxalate in the kidneys, urinary excretion of calcium, oxalate and creatinine in the preventive and curative control rats. In these groups, increased levels of malondialdehyde and depleted levels of antioxidant enzymes, reduced glutathione and catalase were observed. On treatment with the extract, a significant reduction in the deposition of calcium, oxalate and also urinary excretion of calcium, oxalate and creatinine was observed, indicating its antiurolithiatic effect. The extract administration also decreased the extent of lipid peroxidation and enhanced the levels of antioxidant enzymes in the kidneys of urolithic rats, reflecting its antioxidant efficacy against hyperoxaluria induced renal oxidative stress. Results of the present study support the traditional claim of R. tuberosa ischolar_mains in treating renal calculi.Keywords
Ruellia tuberosa, Calcium Oxalate Urolithiasis, Ethylene Glycol/Ammonium Chloride, Oxidative Stress, Antiurolithiatic and Antioxidant Activities.- Evaluation of In-Vitro Anti-Oxidant Activity of Various Extracts of Actinodaphne madraspatana Leaves
Abstract Views :177 |
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Authors
B. Suneetha
1,
K. V. S. R. G. Prasad
2,
B. R. Soumya
3,
P. Deepthi Nishantha
3,
B. Sampath Kumar
3,
D. Rajaneekar
3
Affiliations
1 Department of Biotechnology, Acharya Nagarjuna University, Guntur, IN
2 Padmavathi Mahila Visvavidyalayam, Tirupathi, IN
3 Department of Pharmacology, Malla Reddy Institute of Pharmaceutical Sciences, Maisammaguda, Secunderabad-500 014, Andhra Pradesh, IN
1 Department of Biotechnology, Acharya Nagarjuna University, Guntur, IN
2 Padmavathi Mahila Visvavidyalayam, Tirupathi, IN
3 Department of Pharmacology, Malla Reddy Institute of Pharmaceutical Sciences, Maisammaguda, Secunderabad-500 014, Andhra Pradesh, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 6, No 1 (2014), Pagination: 1-4Abstract
The present study was designed to investigate the in-vitro anti-oxidant activity of methanolic (MEAM), ethyl acetate (EAEAM), chloroform (CEAM) and aqueous (AEAM) extracts of leaves of Actinodaphne madraspatana (AM). MEAM, EAEAM, CEAM and AEAM extracts of leaves were also subjected to the preliminary phytochemical screening. The in-vitro models selected for the present study are DPPH (Di- Phenyl Picryl Hydrazine) free radical scavenging method, Nitric Oxide (NO) free radical scavenging method and Reducing power assay (RPA). Ascorbic acid was used as a standard in these three methods. The first two methods include the direct measurement of free radical scavenging activity whereas the remaining method includes the evaluation of reducing power. IC50 and R2 values of MEAM, EAEAM, CEAM and AEAM were also calculated in DPPH and Nitric Oxide free radical scavenging methods. The preliminary phytochemical screening of MEAM, EAEAM, CEAM and AEAM extracts of leaveshas revealed the presence of various bioactive principles such as tannins, flavonoids, saponins and steroids. From the present study we can propose that MEAM, EAEAM, CEAM and AEAM leaf extracts possess a significant anti-oxidant activity. However, further investigations are to be undertaken in order to elucidate the mechanisms of anti-oxidant activities of leaf extracts of Actinodaphne madraspatana.Keywords
Actinodaphne madraspatana, In-Vitro Antioxidant Activity, DPPH Method, Nitric Oxide Method, Reducing Power Assay.- Evaluation of Antidiabetic and Antioxidant activity of n-Cinnamoyl Metformin Analogues
Abstract Views :252 |
PDF Views:7
Authors
Affiliations
1 Department of Pharmacology, Pulla Reddy Institute of Pharmacy., Hyderabad., Andhra Pradesh, IN
2 Department of Pharmacology, Sri Padmavathi Mahila University, Tirupathi, IN
1 Department of Pharmacology, Pulla Reddy Institute of Pharmacy., Hyderabad., Andhra Pradesh, IN
2 Department of Pharmacology, Sri Padmavathi Mahila University, Tirupathi, IN